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maxima h minus reverse transcriptase (200u/μl) with 5×rt buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher maxima h minus reverse transcriptase (200u/μl) with 5×rt buffer

    Maxima H Minus Reverse Transcriptase (200u/μl) With 5×Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reverse-transcriptase+buffer/maxima+h+minus+reverse+transcriptase/pmc11539149-15-7-10
    Average 90 stars, based on 1 article reviews
    maxima h minus reverse transcriptase (200u/μl) with 5×rt buffer - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile"

    Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

    Journal: iScience

    doi: 10.1016/j.isci.2024.111074


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics

    Related Articles

    Reverse Transcription:

    Article Title: Anti-capsule human monoclonal antibodies protect against hypervirulent and pandrug-resistant Klebsiella pneumoniae
    Article Snippet: .. Reverse transcription (RT) reaction was performed by adding 25 μL per well of a mix containing 1 μL of random hexamer primers (50 ng/mL),1 μL of dNTPs (10 mM), 2 μL 0.1 M DTT, 40 U/μL Rnase OUT, MgCl 2 (25 mM), 5 μL of 5X buffer, 0.25 μL of Superscript IV reverse transcriptase (Invitrogen) and nuclease-free water (DEPC) and RT-PCR conditions were 42°C/10 min, 25°C/10 min, 50°C/ 60 min and 94°C/5 min. After cDNA synthesis, two additional rounds of PCR were performed to obtain the variable regions of the heavy (V H ) and light (V L ) chains. .. Briefly, in the first round of PCR (PCR I) a total volume of 25 μL containing 4 μL of cDNA, 10 μM of VH or 10 μM VL/VK primer mix ( Table S2 ), 0.5 μL of dNTPs (10 mM), 1.5 μL MgCl 2 (25 mM), 5 μL of 5X Kapa Long Range Buffer, and 0.125 μL of Kapa Long Range Polymerase (Sigma) was added in each well and amplified using the following conditions: 95°C/3’, 5 cycles at 95°C/30’’, 57°C/30’’, 72°C/ 30’’ and 30 cycles at 95°C/30’’, 60°C/30’’, 72°C/30’’ and 72°C/2’.

    Article Title: Echovirus 30 in Bulgaria during the European Upsurge of the Virus, 2017–2018
    Article Snippet: For the molecular-based diagnostics, viral RNA was extracted from 400 μL stool suspensions, CSF, or nasopharyngeal swabs by automated extraction with ExiPrep Dx Viral DNA/RNA kit (Bioneer, Daejeon, Republic of Korea) according to the manufacturer instructions. .. For reverse transcription, 10 μL of extracted RNA was denaturated at 95 °C for 5 min. cDNA was generated by adding a 7.5 μL mixture, containing 0.5 μL dNTP mix (25 mM each) (Thermo ScientificTM), 0.5 μL random hexamers (10 μM), 0.5 μL 100 mM Dithiothreitol (DTT), 3.5 μL 5× First strand reaction buffer and 0.5 μL SuperScriptIII reverse transcriptase (Invitrogen, Waltham, MA, USA) at final volume of 17.5 μL. .. The temperature conditions for reverse transcription were as follows: 50 °C for 60 min, followed by 95 °C for 5 min. PanEV primers were used for enterovirus detection as described previously [ ], using OneTaq Hot Start Quick-Load 2× Master Mix (New England Biolabs, Ipswich, MA, USA).

    Article Title: Spatial and Single-Cell Analyses Reveal Heterogeneity of DNAM-1 Receptor–Ligand Interactions That Instructs Intratumoral γδT-cell Activity
    Article Snippet: After washed with 0.1× saline sodium citrate buffer (Thermo Fisher Scientific Inc.) supplemented with 0.05 U/mL RNase inhibitor (NEB), tissue sections placed on the chip were permeabilized using 0.1% pepsin (Sigma-Aldrich) in 0.01 mol/L HCl buffer, incubated at 37°C for 5 minutes before washed with 0.1× SSC buffer supplemented with 0.05 U/mL RNase inhibitor. .. RNA released from the permeabilized tissue and captured by the DNA nanoball was reverse-transcribed overnight at 42°C using SuperScript II (Invitrogen; 10 U/mL reverse transcriptase, 1 mmol/L dNTPs, 1 mol/L betaine solution PCR reagent, 7.5 mmol/L MgCl 2 , 5 mmol/L dithiothreitol (DTT), 2 U/mL RNase inhibitor, 2.5 mmol/L Stereo-seq-template switch oligo (TSO), and 1× First-Strand buffer). ..

    Article Title: Protocol to isolate broadly neutralizing monoclonal antibodies against SARS-CoV-2 from human B cells
    Article Snippet: .. 5× First-Strand Buffer (part of the SuperScript III Reverse Transcriptase) , Thermo Fisher Scientific , Cat#18080044. .. SuperScript III Reverse Transcriptase , Thermo Fisher Scientific , Cat#18080044.

    Random Hexamer:

    Article Title: Anti-capsule human monoclonal antibodies protect against hypervirulent and pandrug-resistant Klebsiella pneumoniae
    Article Snippet: .. Reverse transcription (RT) reaction was performed by adding 25 μL per well of a mix containing 1 μL of random hexamer primers (50 ng/mL),1 μL of dNTPs (10 mM), 2 μL 0.1 M DTT, 40 U/μL Rnase OUT, MgCl 2 (25 mM), 5 μL of 5X buffer, 0.25 μL of Superscript IV reverse transcriptase (Invitrogen) and nuclease-free water (DEPC) and RT-PCR conditions were 42°C/10 min, 25°C/10 min, 50°C/ 60 min and 94°C/5 min. After cDNA synthesis, two additional rounds of PCR were performed to obtain the variable regions of the heavy (V H ) and light (V L ) chains. .. Briefly, in the first round of PCR (PCR I) a total volume of 25 μL containing 4 μL of cDNA, 10 μM of VH or 10 μM VL/VK primer mix ( Table S2 ), 0.5 μL of dNTPs (10 mM), 1.5 μL MgCl 2 (25 mM), 5 μL of 5X Kapa Long Range Buffer, and 0.125 μL of Kapa Long Range Polymerase (Sigma) was added in each well and amplified using the following conditions: 95°C/3’, 5 cycles at 95°C/30’’, 57°C/30’’, 72°C/ 30’’ and 30 cycles at 95°C/30’’, 60°C/30’’, 72°C/30’’ and 72°C/2’.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Anti-capsule human monoclonal antibodies protect against hypervirulent and pandrug-resistant Klebsiella pneumoniae
    Article Snippet: .. Reverse transcription (RT) reaction was performed by adding 25 μL per well of a mix containing 1 μL of random hexamer primers (50 ng/mL),1 μL of dNTPs (10 mM), 2 μL 0.1 M DTT, 40 U/μL Rnase OUT, MgCl 2 (25 mM), 5 μL of 5X buffer, 0.25 μL of Superscript IV reverse transcriptase (Invitrogen) and nuclease-free water (DEPC) and RT-PCR conditions were 42°C/10 min, 25°C/10 min, 50°C/ 60 min and 94°C/5 min. After cDNA synthesis, two additional rounds of PCR were performed to obtain the variable regions of the heavy (V H ) and light (V L ) chains. .. Briefly, in the first round of PCR (PCR I) a total volume of 25 μL containing 4 μL of cDNA, 10 μM of VH or 10 μM VL/VK primer mix ( Table S2 ), 0.5 μL of dNTPs (10 mM), 1.5 μL MgCl 2 (25 mM), 5 μL of 5X Kapa Long Range Buffer, and 0.125 μL of Kapa Long Range Polymerase (Sigma) was added in each well and amplified using the following conditions: 95°C/3’, 5 cycles at 95°C/30’’, 57°C/30’’, 72°C/ 30’’ and 30 cycles at 95°C/30’’, 60°C/30’’, 72°C/30’’ and 72°C/2’.

    cDNA Synthesis:

    Article Title: Anti-capsule human monoclonal antibodies protect against hypervirulent and pandrug-resistant Klebsiella pneumoniae
    Article Snippet: .. Reverse transcription (RT) reaction was performed by adding 25 μL per well of a mix containing 1 μL of random hexamer primers (50 ng/mL),1 μL of dNTPs (10 mM), 2 μL 0.1 M DTT, 40 U/μL Rnase OUT, MgCl 2 (25 mM), 5 μL of 5X buffer, 0.25 μL of Superscript IV reverse transcriptase (Invitrogen) and nuclease-free water (DEPC) and RT-PCR conditions were 42°C/10 min, 25°C/10 min, 50°C/ 60 min and 94°C/5 min. After cDNA synthesis, two additional rounds of PCR were performed to obtain the variable regions of the heavy (V H ) and light (V L ) chains. .. Briefly, in the first round of PCR (PCR I) a total volume of 25 μL containing 4 μL of cDNA, 10 μM of VH or 10 μM VL/VK primer mix ( Table S2 ), 0.5 μL of dNTPs (10 mM), 1.5 μL MgCl 2 (25 mM), 5 μL of 5X Kapa Long Range Buffer, and 0.125 μL of Kapa Long Range Polymerase (Sigma) was added in each well and amplified using the following conditions: 95°C/3’, 5 cycles at 95°C/30’’, 57°C/30’’, 72°C/ 30’’ and 30 cycles at 95°C/30’’, 60°C/30’’, 72°C/30’’ and 72°C/2’.

    Polymerase Chain Reaction:

    Article Title: Anti-capsule human monoclonal antibodies protect against hypervirulent and pandrug-resistant Klebsiella pneumoniae
    Article Snippet: .. Reverse transcription (RT) reaction was performed by adding 25 μL per well of a mix containing 1 μL of random hexamer primers (50 ng/mL),1 μL of dNTPs (10 mM), 2 μL 0.1 M DTT, 40 U/μL Rnase OUT, MgCl 2 (25 mM), 5 μL of 5X buffer, 0.25 μL of Superscript IV reverse transcriptase (Invitrogen) and nuclease-free water (DEPC) and RT-PCR conditions were 42°C/10 min, 25°C/10 min, 50°C/ 60 min and 94°C/5 min. After cDNA synthesis, two additional rounds of PCR were performed to obtain the variable regions of the heavy (V H ) and light (V L ) chains. .. Briefly, in the first round of PCR (PCR I) a total volume of 25 μL containing 4 μL of cDNA, 10 μM of VH or 10 μM VL/VK primer mix ( Table S2 ), 0.5 μL of dNTPs (10 mM), 1.5 μL MgCl 2 (25 mM), 5 μL of 5X Kapa Long Range Buffer, and 0.125 μL of Kapa Long Range Polymerase (Sigma) was added in each well and amplified using the following conditions: 95°C/3’, 5 cycles at 95°C/30’’, 57°C/30’’, 72°C/ 30’’ and 30 cycles at 95°C/30’’, 60°C/30’’, 72°C/30’’ and 72°C/2’.

    Article Title: Spatial and Single-Cell Analyses Reveal Heterogeneity of DNAM-1 Receptor–Ligand Interactions That Instructs Intratumoral γδT-cell Activity
    Article Snippet: After washed with 0.1× saline sodium citrate buffer (Thermo Fisher Scientific Inc.) supplemented with 0.05 U/mL RNase inhibitor (NEB), tissue sections placed on the chip were permeabilized using 0.1% pepsin (Sigma-Aldrich) in 0.01 mol/L HCl buffer, incubated at 37°C for 5 minutes before washed with 0.1× SSC buffer supplemented with 0.05 U/mL RNase inhibitor. .. RNA released from the permeabilized tissue and captured by the DNA nanoball was reverse-transcribed overnight at 42°C using SuperScript II (Invitrogen; 10 U/mL reverse transcriptase, 1 mmol/L dNTPs, 1 mol/L betaine solution PCR reagent, 7.5 mmol/L MgCl 2 , 5 mmol/L dithiothreitol (DTT), 2 U/mL RNase inhibitor, 2.5 mmol/L Stereo-seq-template switch oligo (TSO), and 1× First-Strand buffer). ..

    other:

    Article Title: RAG-seq: NSR-primed and Transposase Tagmentation-mediated Strand-specific Total RNA Sequencing in Single Cells
    Article Snippet: Next, 5 μl RT mixture [2 μl 5× RT buffer, 100 U Maxima H Minus Reverse Transcriptase (Catalog No. EP0751, Thermo Fisher Scientific), 10 U RiboLock RNase inhibitor, 2 μl 5 M Betaine (Catalog No. B0300, Sigma-Aldrich), and 0.25 μl nuclease-free water] was added to the sample tube, and incubated at 25°C for 5 min, 37°C for 15 min, 40°C for 90 min, 70°C for 15 min, and held on at 4°C.

    Article Title: A genome-wide screen identifies silencers with distinct chromatin properties and mechanisms of repression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER S2 cell Pro-seq Serebreni et al.46 GEO: GSE181257 S2 cell STARR-seq Zabidi et al.43 GEO: GSE57876 S2 cell enhancer variant STARR-seq Reiter et al.28 GEO: GSE211659 S2 cell DHS-seq Arnold et al.41 GEO: GSE40739 S2 cell ATAC-seq Albig et al.59 GEO: GSE119708 S2 cell MNase-seq Ramachandran et al.60 GEO: GSE98351 S2 cell G9a ChIP-seq Ma cinkovi c et al.84 ENA: ERX3548052 S2 cell Su(Hw) ChIP-seq Chen et al.67 GEO: GSE27679 S2 cell H3K9me2 ChIP-seq modENCODE Consortium39 GEO: GSE47229 S2 cell H3K9me3 ChIP-seq Gerland et al.121 GEO: GSE86106 S2 cell H3K27me3 ChIP-seq Herz et al.122 GEO: GSE41440 S2 cell H3K27ac ChIP-seq Rickels et al.108 GEO: GSE81795 S2 cell H3K27me1 ChIP-seq Rickels et al.108 GEO: GSE81795 UniProt reference proteomes (rpg35) UniProt Consortium78 https://www.uniprot.org/ RNA-seq across developmental stages Graveley et al.123; modENCODE project SRA: SRP001065, gene RPKM values obtained from Flybase (gene_rpkm_report_fb_2017_05.tsv) Flyatlas gene expression dataset Chintapalli et al.69 https://motif.mvls.gla.ac.uk/flyatlas/ Experimental Models: Cell Lines and Fly Strains D. melanogaster Schneider S2 cells ThermoFisher R69007 S2OsTir1 Hendy et al.66 N/A S2OsTir1-CG11247/Saft-AID-cl1 This Study N/A S2OsTir1-CG11247/Saft-AID-cl2 This Study N/A S2OsTir1-Su(Hw)-AID This Study N/A S2OsTir1-Phaser-AID This Study N/A S2OsTir1-G9a-AID This Study N/A S2OsTir1-Lft-deltaDLM3-cl1 This Study N/A S2OsTir1-Lft-deltaDLM3-cl2 This Study N/A S2OsTir1-Lft-deltaDLM3-cl3 This Study N/A S2TurboID-GFP This Study N/A S2CG11247/Saft-TurboID This Study N/A w;; CG11247[indel1]/TM3,Sb; (Saft mut 1) This Study, available from VDRC VDRC-ID 312158 w;; CG11247[indel2]/TM3,Sb; (Saft mut 2) This Study, available from VDRC VDRC-ID 312159 w;;; (wildtype) Received from Baumgartner et al.124; originally from Susan Celniker iso1 wildtype Oligonucleotides Primers for DNA motif and protein sequence mutagenesis This Study Table S2 Primers for cell line engineering and genotyping This Study Table S2 Primers for qPCRs This Study Table S2 Recombinant DNA pAc-sgRNA-Cas9 Serebreni et al.46; Hendy et al.66 Addgene #49330 pCRIS-PITChv2-FBL Serebreni et al.46; Hendy et al.66 Addgene #63672 Cas9 expression plasmid Addgene #69090 silencer-seq sgl vector This Study Table S2 silencer-seq ham vector This Study Table S2 pGL3 luciferase reporter vector Promega E1751 pRL-ubi63E Arnold et al.41 Addgene #74280 pGL3-sgl-DSCP-luc+ This Study Table S2 (Continued on next page) e3 Molecular Cell 84, 1–19.e1–e14, December 5, 2024

    Article Title: Circular RNA identification and implication of a role of circAGO3 in the infection of white spot syndrome virus in Pacific white shrimp.
    Article Snippet: White spot syndrome is one of the most serious diseases in shrimp aquaculture.. Studies and research are needed to protect shrimp from this disease.. Circular RNA (circRNA) is one of RNA molecules involved in the regulations of various physiological processes.

    Generated:

    Article Title: Echovirus 30 in Bulgaria during the European Upsurge of the Virus, 2017–2018
    Article Snippet: For the molecular-based diagnostics, viral RNA was extracted from 400 μL stool suspensions, CSF, or nasopharyngeal swabs by automated extraction with ExiPrep Dx Viral DNA/RNA kit (Bioneer, Daejeon, Republic of Korea) according to the manufacturer instructions. .. For reverse transcription, 10 μL of extracted RNA was denaturated at 95 °C for 5 min. cDNA was generated by adding a 7.5 μL mixture, containing 0.5 μL dNTP mix (25 mM each) (Thermo ScientificTM), 0.5 μL random hexamers (10 μM), 0.5 μL 100 mM Dithiothreitol (DTT), 3.5 μL 5× First strand reaction buffer and 0.5 μL SuperScriptIII reverse transcriptase (Invitrogen, Waltham, MA, USA) at final volume of 17.5 μL. .. The temperature conditions for reverse transcription were as follows: 50 °C for 60 min, followed by 95 °C for 5 min. PanEV primers were used for enterovirus detection as described previously [ ], using OneTaq Hot Start Quick-Load 2× Master Mix (New England Biolabs, Ipswich, MA, USA).



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    Image Search Results


    Journal: iScience

    Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

    doi: 10.1016/j.isci.2024.111074

    Figure Lengend Snippet:

    Article Snippet: Maxima H Minus Reverse Transcriptase (200U/μL) with 5×RT Buffer , ThermoFisher , Cat# EP0751.

    Techniques: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics